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KMID : 1134820160450081107
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Journal of the Korean Society of Food Science and Nutrition
2016 Volume.45 No. 8 p.1107 ~ p.1113
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Effect of Acacia catechu Extract on 3T3-L1 Preadipocyte Differentiation
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Kim Dong-Gyu
Kang Min-Jung
Suh Hwa-Jin
Kwon Oh-Oun
Shin Jung-Hye
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Abstract
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The purpose of this study was to investigate the effects of catechu water extract on adipogenesis in 3T3-L1 adipocytes. 3T3-L1 preadipocytes were differentiated with adipogenic regents by incubation for 9 days in the absence or presence of catechu extract ranging from 1∼200 μg/mL. The effect of catechu extracts on cell proliferation of 3T3-L1 preadipocytes was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The effect of catechu extracts on 3T3-L1 differentiation was examined by measuring intracellular lipid droplet and triglyceride contents. These results were obtained from preadipocyte proliferation and adipocyte differentiation of 3T3-L1. Catechu extracts inhibited lipid accumulation and remarkably decreased triglyceride contents in 3T3-L1 preadipocytes at a concentration showing no cytotoxicity. The anti-adipogenic effects of catechu appeared to be mediated by significant down-regulation of expression of peroxisome proliferator-activated receptor γ, CCAAT/enhancer-binding protein α, and sterol regulatory element-binding protein 1c proteins apart from expression of hormone-sensitive lipase. We suggest that catechu extracts significantly inhibit adipogenesis and can be used for regulation of obesity.
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KEYWORD
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adipogenesis, triglyceride, PPARγ, C/EBPα, SREBP-1
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